anti-EEF1A1 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-EEF1A1 antibody; EEF1A1;
The EEF1A1 eef1a1 (Catalog #MBS625962) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EEF1A1, NT (Elongation Factor 1-alpha 1, EF-1-alpha-1, Eukaryotic Elongation Factor 1 A-1, Elongation Factor 1 A-1, eEF1A-1, Elongation Factor Tu, EF-Tu, Leukocyte Receptor Cluster Member 7, LENG7, EF1A, EEF1A) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s EEF1A1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB).Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100. Researchers should empirically determine the suitability of the EEF1A1 eef1a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The EEF1A1 eef1a1 product has the following accession number(s) (GI #4503471) (NCBI Accession #NP_001393.1) (Uniprot Accession #P68104). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the EEF1A1, NT (Elongation Factor 1-alpha 1, EF-1-alpha-1, Eukaryotic Elongation Factor 1 A-1, Elongation Factor 1 A-1, eEF1A-1, Elongation Factor Tu, EF-Tu, Leukocyte Receptor Cluster Member 7, LENG7, EF1A, EEF1A) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of EEF1A1 Antibody (N-term) in Y79, T47D cell line lysates (35ug/lane). EEF1A1 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human brain with EEF1A1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS) (Flow cytometric analysis of NCI-H292 cells using EEF1A1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Testing Data (With 293T cell line lysate,resolved proteins were electrophoretically transferred to PVDF membrane and incubated sequentially with primary antibody EF1A1(1:500, 4°C,overnight ) and horseradish peroxidase–conjugated second antibo dy(rabbit). After washing, the bound antibody complex was detected using an ECL chemiluminescence reagent and XAR film (Kodak).)
EEF1A1 is an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome.