anti-DOK2 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-DOK2 antibody; DOK2;
The DOK2 dok2 (Catalog #MBS629475) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DOK2, phosphorylated (Tyr299) (Docking Protein 2, Downstream Of Tyrosine Kinase 2, p56(dok-2), p56Dok-2) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s DOK2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC).Suitable for use in Western Blot and Immunohistochemistry.
Dilution: Western Blot:1:500-1:1,000
Immunohistochemistry:1:50-1:100. Researchers should empirically determine the suitability of the DOK2 dok2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The DOK2 dok2 product has the following accession number(s) (GI #41406050) (NCBI Accession #NP_003965.2) (Uniprot Accession #O60496). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Docking proteins interact with receptor tyrosine kinases and mediate particular biological responses using signal transduction. Dok-2 acts as a multiple docking protein downstream of receptor or non-receptor tyrosine kinases. By this mechanism it acts to negatively regulate signal transduction and cell proliferation controlled by cytokines in a feedback loop. Dok-2 is highly expressed in cells and tissues of hematopoietic origin as well as in lung. Expression of bcr/abl induces additional tyrosine phosphorylation of the Dok1 and Dok2 proteins and their association with Ras-GAP. Thus, it is suspected that DOK association regulates GAP activity toward Ras and that the Dok proteins serve as mediators of bcr-abl signaling. The role of Dok proteins in bcr-abl regulation may also be implicated in chronic myelogenous leukemia (CML), which is characterized by a Philadelphia chromosome translocation t(9;22). Such a mutation would result in a p210-bcr/abl chimeric protein-tyrosine kinase which has been found in many CML cases.
Immunogen: Synthetic phosphopeptide corresponding to human p56Dok-2 around the phosphorylation site of tyrosine 299 (G-E-YP-A-V). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing DOK2 are readily searchable from our website. Different antibodies against the same target such as DOK2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Blood, Bone, Kidney, Liver, Lung, Spleen, Thymus tissues are correlated with this protein.