anti-DOK2 antibody product blog
Tags: Antibody; Polyclonal Antibody; Dok2, p56; anti-DOK2 antibody; DOK2;
The DOK2 dok2 (Catalog #MBS615420) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Dok2, p56, phosphorylated (Tyr351) (Docking Protein, Downstream of Tyrosine Kinase, Dok-R, p56Dok2, FRIP, rasGAP-associated docking protein) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Dok2, p56 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).Suitable for use in Western Blotting.
Dilution: Western Blot: 1:1000. Researchers should empirically determine the suitability of the DOK2 dok2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The DOK2 dok2 product has the following accession number(s) (GI #41406050) (NCBI Accession #NP_003965.2) (Uniprot Accession #O60496). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Dok2, p56, phosphorylated (Tyr351) (Docking Protein, Downstream of Tyrosine Kinase, Dok-R, p56Dok2, FRIP, rasGAP-associated docking protein) with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of cell extracts from Jurkat cells over expressing p56Dok-2 proteins untreated or stimulated with anti-CD2 antibody using MBS615420 or D8080-02 (lower).)
Docking proteins are substrates of tyrosine kinases and function in the recruitment and assembly of specific signal transduction molecules. There are five members in p62dok family including p62Dok (Dok-1), p56Dok-2 (Dok-2, or DoK-R), Dok-3, Dok-4 and Dok-5 (1-3), which are characterized by the presence of an amino-terminal PH domain, a central PTB domain and numerous potential sites of tyrosine phosphorylation. Tyrosine phosphorylation of p56Dok-2 occurs upon stimulation of cells with a variety of stimuli or in cells transformed by oncogenic tyrosine kinases such as v-Src and Bcr-Abl (3-5). Very little is known about p56Dok-2 function and the role of phosphorylation in the modulation of these functions. Based on the presence of several signaling domains (PH, PTB domain, tyrosine residue and proline-rich regions), it has been proposed that they act as docking proteins that link RTKs to signal transduction pathways. p56Dok-2 has been proposed to be a negative regulator of cytokine-induced proliferation in T cells (5). It was demonstrated that phosphorylated Tyr351 mediates an association with the SH2 domain of Nck (4).
Signals from most growth factors and cytokines are transduced by receptor tyrosine kinases or non-receptor tyrosine kinases. Activated tyrosine kinases phosphorylate their substrates, which mediate the cellular response to extracellular stimuli. A long-sought major substrate termed p62dok (downstream of tyrosine kinase) for many tyrosine kinases including c-kit, v-abl, v-Fps, v-Src, v-Fms, and activated EGF, PDGF, IGF, VEGF and insulin receptors was identified recently from human and mouse by several laboratories. Upon phosphorylation, p62dok forms a complex with the ras GTPase-activating protein (RasGAP). p62dok represents a new family with very recently identified p56dok. Human DOK1 is a 481 amino acid protein.