anti-DMPO antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-DMPO antibody; DMPO;
The DMPO n/a (Catalog #MBS622873) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s DMPO can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC).Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: Western Blot: 1:5,000
ELISA: 1:5,000 to 1:10,000
Immunohistochemistry: 1:1,000 (subject to optimization). Researchers should empirically determine the suitability of the DMPO n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The DMPO n/a product has the following accession number(s) (GI #51242284). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Free radicals are highly reactive and generally short-lived species. Hence, most studies of free radicals have been restricted to analysis of the end products formed by their interaction with cellular biomolecules. One technology that has permitted direct study of free radicals is electron spin resonance (ESR). For very short lived radicals, spin traps have been empolyed to generate longer lived radicals that are more amenable to ESR analysis. The most widely used of these spin traps is 5,5-dimethyl-1-pyrroline-N-oxide (DMPO). The reaction of DMPO with a protein radical gives rise to a nitroxide radical which can be disproportionate to hydrozylamine and nitrone adducts. Of these species, only the nitrone adduct can be considered stable. It has been shown that our DMPO antibody will specifically react with radicals in protein.
DMPO reagent can be employed for the immunochemical identifcation and characterization of nitrone adducts that result from the reaction of protein radicals with DMPO by immuno-spin trapping.
Immunogen: 5,5-dimethyl-1-pyrroline-N-oxide (DMPO)-octanoic acid adduct (ovalbumin). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing DMPO are readily searchable from our website. Different antibodies against the same target such as DMPO may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Bone, Brain, Connective Tissue, Ear, Embryonic Tissue, Heart, Kidney, Muscle, Umbilical Cord, Vascular tissues are correlated with this protein. DMPO also interacts with the following gene(s): FLNA.