|The Dimethyl-Histone H3 (Lys27) n/a (Catalog #MBS462179) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti Dimethyl-Histone H3 (Lys 27) reacts with Human, Rat, Mouse, Chicken, Bovine and may cross-react with other species as described in the data sheet. MyBioSource\'s Dimethyl-Histone H3 (Lys27) can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot, ChIP, Immunohistochemistry.
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 1:100. Researchers should empirically determine the suitability of the Dimethyl-Histone H3 (Lys27) n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rabbit anti Dimethyl-Histone H3 (Lys 27) with the following immunoassay(s):
Histones are basic nuclear proteins that together with DNA make up the nucleosome structure in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The methylation of specific residues in the histone tails is a central modification for regulatingepigenetic transitions in chromatin. Whereas methylation of histone H3 on lysine 4, 36, and 79 has been linked with gene activation, methylation of H3 on lysines 9, 27 and histone H4 on lysine 20 is associated with heterochromatin and some repressed genes with euchromatin. Modified lysine residues can exist in a mono-, di-, or tri-methylated state, while the arginine residues can be mono- or di-methylated. Histone H3 Lys4 trimethylation (H3-K4me3) is a conserved mark of actively transcribed chromatin. This antibody is specific for histone H3 tri-methylated at K4. The sequence is found in all mammals and a wide range of species, including D. melanogaster, Arabidopsis, Chicken and Xenopus. The antibody will react with any of the above species where the trimethylation modification is present.
Antigen Preparation: A synthetic peptide containing AR[Me2-K]ST in which Me2-K corresponds to Dimethyl lysine 27 of human histone H3. Positive Control: 3T3 Nuclear Extract
Cellular Location: Nuclear. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Dimethyl-Histone H3 (Lys27) are readily searchable from our website. Different antibodies against the same target such as Dimethyl-Histone H3 (Lys27) may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.