anti-DFF40 (NT) antibody product blog
Tags: Antibody; DFF40 (NT); anti-DFF40 (NT) antibody;
The DFF40 (NT) n/a (Catalog #MBS194066) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s DFF40 (NT) can be used in a range of immunoassay formats including, but not limited to, Immunoblot.Immunoblotting: use at 1:500-1:2,000 dilution.
Positive control: Whole cell lysate of K562 or Jurkat cells. Researchers should empirically determine the suitability of the DFF40 (NT) n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain-containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse Dnase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxy-ribonuclease). The human homologue of mouse CAD was more recently identified and termed CPAN, DFF40, and human CAD. DFF45/ICAD is the inhibitory protein of DFF40/CAD with which it forms complexes. Upon cleavage by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of DFF40/CAD, which causes DNA degradation, is the hallmark of apoptotic cell death.
Antigen: Peptide corresponding to aa 3-18 of human DFF40. Stabilizer: None
Preservatives: 0.02% sodium azide.
Dilutions Instructions: Dilute in PBS or medium which is identical to that used in the assay system. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing DFF40 (NT) are readily searchable from our website. Different antibodies against the same target such as DFF40 (NT) may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.