anti-DENN antibody product blog
Tags: Antibody; Polyclonal Antibody; DENN / MADD; anti-DENN antibody; DENN;
The DENN madd (Catalog #MBS240336) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Human DENN / MADD reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s DENN / MADD can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB).ICC, IHC-P (2.5 ug/ml), WB (1:250 - 1:500)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib.. Researchers should empirically determine the suitability of the DENN madd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The DENN madd product has the following accession number(s) (GI #209862994) (NCBI Accession #NP_003673.3) (Uniprot Accession #Q8WXG6). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rabbit Polyclonal to Human DENN / MADD with the following immunoassay(s):
Immunohistochemistry (IHC) (Anti-MADD antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Immunofluorescence (IF) (Immunofluorescence of MADD in HeLa cells with MADD antibody at 20 ug/ml.)
Immunocytochemistry (ICC) (Immunocytochemistry of MADD in human spleen tissue with MADD antibody at 10 ug/ml.)
Western Blot (WB) (Western blot of MADD in whole cell lysates from the indicated cell lines with MADD antibody at 1:250 dilution.)
MAP kinase-activating death domain protein (MADD) was initially identified as the type 1 tumor necrosis factor receptor (TNFR1) associated protein though their death domains. Overexpression of MADD activates MAP kinases ERK and JNK and induces the phosphorylation of cytosolic phospholipase A2 (1). MADD shares 98% identity with DENN (for differentially expressed in neoplastic vs. normal cells), which was recently identified as a substrate for c-jun N-terminal kinase 3 (JNK3).
Target Species: Human
Immunogen Description: Synthetic peptide from human MADD. Immunogen Type: Synthetic peptide
Immunogen: DENN / MADD antibody was raised against synthetic peptide from human MADD.
Antigen Modification: C-Terminus. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing DENN are readily searchable from our website. Different antibodies against the same target such as DENN may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.