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anti-DDX3X antibody product blog

Posted on 2013-12-15 15:42:47 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; DDX3X; anti-DDX3X antibody;
The DDX3X ddx3x (Catalog #MBS120212) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse monoclonal antibody Anti-Human DDX3X reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s DDX3X can be used in a range of immunoassay formats including, but not limited to, Dot Blot (DB), Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB).
WB: 0.2-2 ug/ml
IP: 100-500 ug/sample
ICC: 2-100 ug/ml. Researchers should empirically determine the suitability of the DDX3X ddx3x for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The DDX3X ddx3x product has the following accession number(s) (GI #698980011) (NCBI Accession #NP_001347.3). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Mouse monoclonal antibody Anti-Human DDX3X with the following immunoassay(s):
Quality Control (Western blot analysis of immunized recombinant protein, using anti-DDX3X monoclonal antibody.)
Quality Control DDX3X.

Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control #2 DDX3X.

Western Blot (WB) (Detection of DDX3X by Western blot.

Samples: Whole cell lysate from human HeLa (H, 25 ug), mouse NIH3T3 (M, 50 ug) and rat F2408 (R, 50 ug) cells. [Lot No. 2253C5a-1]

Predicted molecular weight: 73 kDa)
Western Blot (WB) DDX3X.

Immunoprecipitation (IP) (Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-DDX3X mAb. [Lot No. 2253C5a-1]

Predicted molecular weight: 73 kDa)
Immunoprecipitation (IP) DDX3X.

Immunocytochemistry (ICC) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-DDX3X mAb. [Lot No. 2253C5a-1])
Immunocytochemistry (ICC) DDX3X.

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which interacts specifically with hepatitis C virus core protein resulting a change in intracellular location. This gene has a homolog located in the nonrecombining region of the Y chromosome. The protein sequence is 91% identical between this gene and the Y-linked homolog. [NCBI Entrez Gene Summary].

Preparation: This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Immunogen: Recombinant.
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