anti-DDR2 antibody product blog
Tags: Antibody; Monoclonal Antibody; DDR2; anti-DDR2 antibody;
The DDR2 ddr2 (Catalog #MBS850146) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The DDR2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s DDR2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA).WB: 1:500-1:2000
IHC: 1:200-1:1000
IF: 1:200-1:1000
ELISA: 1:10000. Researchers should empirically determine the suitability of the DDR2 ddr2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The DDR2 ddr2 product has the following accession number(s) (GI #62420886) (NCBI Accession #NP_001014796.1) (Uniprot Accession #Q16832). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the DDR2 Antibody with the following immunoassay(s):
Western Blot (WB) (Figure 1: Western blot analysis using DDR2 mouse mAb against truncated DDR2 recombinant protein.)
Immunohistochemistry (IHC) (Figure 2: Immunohistochemical analysis of paraffin-embedded human skeletal musle tissues using DDR2 mouse mAb.)
Immunofluorescence (IF) (Figure 3: Confocal immunofluorescence analysis of A549 cells using DDR2 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.)
DDR2 (discoidin domain receptor family, member 2) is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. Receptor tyrosine kinases (RTKs) play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation, and metabolism. In several cases the biochemical mechanism by which RTKs transduce signals across the membrane has been shown to be ligand induced receptor oligomerization and subsequent intracellular phosphorylation. This autophosphorylation leads to phosphorylation of cytosolic targets as well as association with other molecules, which are involved in pleiotropic effects of signal transduction. RTKs have a tripartite structure with extracellular, transmembrane, and cytoplasmic regions. This gene encodes a member of a novel subclass of RTKs and contains a distinct extracellular region encompassing a factor VIII-like domain. Alternative splicing in the 5\' UTR results in multiple transcript variants encoding the same protein.
Immunogen: Purified recombinant fragment of human DDR2 expressed in E. Coli. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing DDR2 are readily searchable from our website. Different antibodies against the same target such as DDR2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.