anti-CYP3A4 antibody product blog
Tags: Antibody; anti-CYP3A4 antibody; CYP3A4; Polyclonal Antibody;
The CYP3A4 cyp3a4 (Catalog #MBS624279) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CYP3A4, ID (Cytochrome P450 3A4, Quinine 3-monooxygenase, CYPIIIA4, Nifedipine Oxidase, Cytochrome P450 3A3, CYPIIIA3, Cytochrome P450 HLp, Taurochenodeoxycholate 6-alpha-hydroxylase, Cytochrome P450 NF-25, Cytochrome P450-PCN1, Albendazole Monooxygenase, reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CYP3A4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB).Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100. Researchers should empirically determine the suitability of the CYP3A4 cyp3a4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CYP3A4 cyp3a4 product has the following accession number(s) (GI #322302351) (NCBI Accession #NP_001189784.1) (Uniprot Accession #P08684). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the CYP3A4, ID (Cytochrome P450 3A4, Quinine 3-monooxygenase, CYPIIIA4, Nifedipine Oxidase, Cytochrome P450 3A3, CYPIIIA3, Cytochrome P450 HLp, Taurochenodeoxycholate 6-alpha-hydroxylase, Cytochrome P450 NF-25, Cytochrome P450-PCN1, Albendazole Monooxygenase, with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of CYP3A4 Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). CYP3A4 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC) (CYP3A4 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CYP3A4 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS) (CYP3A4 Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
CYP3A4, is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs which are are used today, including acetaminophen, codeine, cyclosporin A, diazepam and erythromycin. The enzyme also metabolizes some steroids and carcinogens.