|The CYCLIN D1 ccnd1 (Catalog #MBS212298) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI CYCLIN D1 reacts with Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s CYCLIN D1 can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, ELISA (EIA), Flow cytometry (FC/FACS)*, Immunoprecipitation (IP), Immunohistology Paraffin*, Western Blot (WB).
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry: Maximum Dilution: Neat; Application Note: Membrane permeabilization is required for this application. MyBioSource recommend the use of Leucoperm
Immunohistology - Paraffin: Minimum Dilution: 1/1000; Maximum Dilution: 1/10000; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Western Blotting: Maximum Dilution: 1/500. Researchers should empirically determine the suitability of the CYCLIN D1 ccnd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CYCLIN D1 ccnd1 product has the following accession number(s) (GI #16950655) (NCBI Accession #NP_444284.1) (Uniprot Accession #P24385). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Mouse anti Cyclin D1 antibody, clone CD1.1 recognizes cyclin D1, a 295 amino acid, 36kDa nuclear protein which belongs to the highly conserved cyclin family. D type cyclins are predominantly expressed in the G1 phase of the cell cycle. Cyclin D1 study is of interest in various forms of malignancy and over expression has been linked to poor prognosis (Bartkova et al. 1995).
Perservative Stabilisers: 0.09% Sodium Azide
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant. Histology Positive Control Tissue: Small intestine, colon
Buffer Solution: Phosphate buffered saline
Target Species: Human. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CYCLIN D1 are readily searchable from our website. Different antibodies against the same target such as CYCLIN D1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Blood, Bone, Brain, Liver, Lung, Lymph, Lymph Node, Muscle, Prostate, Vascular tissues are correlated with this protein. CYCLIN D1 also interacts with the following gene(s): CDK2, CDK4, CDK6, CDKN1A, CDKN1B, ESR1, PCNA, RB1, STAT3, UBC.