anti-cx47.1 antibody product blog
Tags: Antibody; Monoclonal Antibody; Connexin 47; anti-cx47.1 antibody; cx47.1;
The cx47.1 cx47.1 (Catalog #MBS601521) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Connexin 47 reacts with Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s Connexin 47 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF).Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation.
Dilution: Immunofluorescence: 1-3ug/ml
Western Blotting: 1-3ug/ml
Immunoprecipitation: 5-10ug/ml. Researchers should empirically determine the suitability of the cx47.1 cx47.1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The cx47.1 cx47.1 product has the following accession number(s) (GI #52219002) (NCBI Accession #NP_001004574.1) (Uniprot Accession #Q66HV7). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Connexin 47 with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of (A) mouse thalamus homogenates and (B) connexin47 cDNA-transfected HeLa cells using MBS601521)
Intercellular communication through gap junctions (connexins) plays an important role in a variety of cellular processes including homeostasis, morphogenesis, cell differentiation and growth control.1-3 In rodents, 15 connexin genes have been described4,9. Specific cell types in the brain express specific types of connexins; expression patterns coincide with tissue compartmentalization and function. These compartments change during development5. Reduction or alteration in the levels or types of connexin expressed in a given cell type has been found to correlate with tumor progression and metastasis10. The coding sequence of the mouse gap junction Cx47 has 49% homology with mouse Cx45; both are located on chromosome 11 in the mouse genome6,8. Cx47 has been observed to express mainly in the gray matter of the central nervous system, specifically in several types of hippocampal, cortical, cerebellar and spinal cord neurons8. Functional gap junctions are involved in tissue homeostasis of ions, metabolites and second messenger molecules10. Between neuronal cells, these functions play a role in synchronizing oscillations of certain cell clusters and could impact.
Immunogen: Synthetic peptide derived from the C-terminal region of the mouse Connexin 47 protein. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing cx47.1 are readily searchable from our website. Different antibodies against the same target such as cx47.1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.