|The CTTN ems1 (Catalog #MBS615537) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Cortactin, phosphorylated (Tyr466) reacts with Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s Cortactin can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB).
Suitable for use in Western Blot.
Dilution: Western Blot: 0.1-1ug/ml. Researchers should empirically determine the suitability of the CTTN ems1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CTTN ems1 product has the following accession number(s) (GI #40645044) (Uniprot Accession #Q76MU0). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Cortactin is an 80/85kD cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein-tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino-terminal tandem repeats of an unique 37aa sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an a helical structure followed by a proline-rich region. Between the proline-rich region and the SH3 domain, there are Src phosphorylation sites at tyrosines 421, 466 and 482 (421, 470 and 486 in the human sequence) that when phosphorylated, attenuate the cross-linking of F-actin into meshworks.
Immunogen: Synthetic phosphopeptide corresponding to mouse cortactin that contains tyrosine 466 (tyrosine 470 in the human sequence). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CTTN are readily searchable from our website. Different antibodies against the same target such as CTTN may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Blood, Bone, Brain, Embryonic Tissue, Kidney, Lung, Lymph Node, Muscle, Testis, Vascular tissues are correlated with this protein. CTTN also interacts with the following gene(s): ACTR3, ASAP1, FER, GRB2, HAX1, MET, PXN, SHANK2, SRC, SYK. Adenocarcinoma, Breast Neoplasms, Carcinoma, Carcinoma, Squamous Cell, Cell Transformation, Neoplastic, Lung Neoplasms, Neoplasm Metastasis, Neoplasms, Neoplasms, Experimental, Skin Diseases are some of the diseases may be linked to Cortactin, phosphorylated (Tyr466). The following patways have been known to be associated with this gene.