anti-CTLA-4 antibody product blog
Tags: Antibody; Monoclonal Antibody; CTLA-4; anti-CTLA-4 antibody;
The CTLA-4 ctla4 (Catalog #MBS520258) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Human CTLA-4, FITC, (clone: A3.4H2.H12), (mouse IgG2ak) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CTLA-4 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS).Flow Cytometry:
Cell concentration: 1x106cells per tests
Antibody Concentration Used: 1.0 ug/106 cells. Researchers should empirically determine the suitability of the CTLA-4 ctla4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CTLA-4 ctla4 product has the following accession number(s) (GI #21361212) (NCBI Accession #NP_005205.2) (Uniprot Accession #P16410). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-Human CTLA-4, FITC, (clone: A3.4H2.H12), (mouse IgG2ak) with the following immunoassay(s):
Testing Data
Human CTLA-4 (also known as Cytotoxic T lymphocyte-associated antigen-4, CD152) is a structural homologue of T cell co-stimulatory receptor CD28. CTLA-4 binds to the same ligands (CD80 and CD86) as CD28 but with much higher avidity. It is expressed at very low levels on the cell surface of activated T cells, and is found primarily in the post-Golgi or endosomal compartments of the cell.
There is a high degree of overall homology between human and mouse CTLA-4 (>70%), and the cytoplasmic domains of the human, mouse and rabbit sequences are completely conserved. CD152 antibodies have shown both stimulatory and negative regulatory roles in functional experiments. This antibody is suitable for use in Intracellular Flow Cytometry.
Hybridoma Production: Immunization:
Immunogen: Activated T cells
Donor: Balb/c mouse
Fusion Partner:
SP 2/0 myeloma. Presentation: 100 �ug FITC conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CTLA-4 are readily searchable from our website. Different antibodies against the same target such as CTLA-4 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. CTLA-4 also interacts with the following gene(s): CD276, CD80, CD86, FOXP3, FYN, LCK, LYN, PTPN11.