anti-CST8 antibody product blog
Tags: Antibody; Polyclonal Antibody; Cystatin 8; anti-CST8 antibody; CST8;
The CST8 cst8 (Catalog #MBS421965) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Cystatin 8 (aa 23-34) Antibody reacts with Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Cystatin 8 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB-Trf).Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: In transfected HEK293 transiently expressing Human CST8 (myc and DYKDDDDK tagged), a band of approx.23kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc tag antibody. Recommended concentration, 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the CST8 cst8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CST8 cst8 product has the following accession number(s) (GI #4885163) (NCBI Accession #NP_005483.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
DPKKNETGVL RK.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Goat anti-Cystatin 8 (aa 23-34) Antibody with the following immunoassay(s):
Western Blot (WB) (HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CST8 (RC210130) with C-terminal MYC tag probed (0.3ug/ml) in Lane An and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK294 probed (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.)
Immunogen: Peptide with sequence C-DPKKNETGVLRK, from the internal region of the protein sequence according to NP_005483.1.
Epitope: Internal region. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CST8 are readily searchable from our website. Different antibodies against the same target such as CST8 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Carcinoma, Hepatocellular, Cardiovascular Diseases, Disease Models, Animal, Infertility, Male, Liver Diseases, Liver Neoplasms are some of the diseases may be linked to Goat anti-Cystatin 8 (aa 23-34) Antibody. Testis tissues are correlated with this protein.