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anti-CSPG4 antibody product blog

Posted on 2014-03-18 20:41:42 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; CSPG4; anti-CSPG4 antibody;
The CSPG4 cspg4 (Catalog #MBS128611) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CSPG4 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s CSPG4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC).
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200. Researchers should empirically determine the suitability of the CSPG4 cspg4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The CSPG4 cspg4 product has the following accession number(s) (GI #296434468) (NCBI Accession #Q6UVK1.2) (Uniprot Accession #Q6UVK1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the CSPG4 Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using CSPG4 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.)
Western Blot (WB) CSPG4.

A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.

Species: Human
Route: Synthetic Peptide. Immunogen: A synthetic peptide of human CSPG4
Calculated Molecular Weight: 251kDa. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CSPG4 are readily searchable from our website. Different antibodies against the same target such as CSPG4 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Bone, Brain, Connective Tissue, Embryonic Tissue, Eye, Lung, Lymph Node, Nerve, Placenta, Skin tissues are correlated with this protein. CSPG4 also interacts with the following gene(s): BGN, CHST11, DCN. Adenocarcinoma, Breast Neoplasms, Carcinoma, Cell Transformation, Neoplastic, Lung Neoplasms, Necrosis, Neoplasm Metastasis, Neoplasms, Experimental, Nervous System Diseases, Pancreatic Neoplasms are some of the diseases may be linked to CSPG4 Polyclonal Antibody.
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