anti-CRP antibody product blog
Tags: Antibody; CRP; Monoclonal Antibody; anti-CRP antibody;
The CRP crp (Catalog #MBS200044) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CRP antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s CRP can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS).WB: 1:1,000 ~ 2,000.
Recommended starting dilution is 1:1,000. Researchers should empirically determine the suitability of the CRP crp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CRP crp product has the following accession number(s) (GI #55770842) (NCBI Accession #NP_000558) (Uniprot Accession #P02741). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the CRP antibody with the following immunoassay(s):
Western Blot (WB) (Cell lysates of NIH-3T3, HepG2, Jurkat, HeLa and SH-SY5Y (each 40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRP (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Immunofluorescence (IF) (ICC/IF analysis of CRP in HepG2 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CRP antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
C-reactive protein (CRP) is an acute-phase protein with a well-known association with infection and other inflammatory conditions. Mild elevations in C-reactive protein concentration predict myocardial infarction, stroke, and vascular death in a variety of clinical settings. Despite the lack of specific evidence that C-reactive protein levels are independently associated with cardiovascular risk in patients with hypertension, the prognostic value of C-reactive protein has proven to be complementary to that of blood pressure values.
Antigen Species: Human
Immunogen: Recombinant human CRP (19-224aa) purified from E. coli. Clone Note: Anti-human CRP mAb, clone 5A9, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CRP protein. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CRP are readily searchable from our website. Different antibodies against the same target such as CRP may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.