anti-CREB1 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-CREB1 antibody; CREB1; phospho CREB1;
The CREB1 creb1 (Catalog #MBS551068) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti phospho CREB1 Polyclonal reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s phospho CREB1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), IHC (Immunohistochemistry), ELISA (EIA).ELISA: Use at a concentration range of 1-2ug/ml.
Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml.
IHC:A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml. Researchers should empirically determine the suitability of the CREB1 creb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CREB1 creb1 product has the following accession number(s) (GI #4758054) (NCBI Accession #NP_004370.1) (Uniprot Accession #P16220). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rabbit anti phospho CREB1 Polyclonal with the following immunoassay(s):
Immunohistochemistry
The ATF/CREB family consists of transcription factors that function through binding to the cAMP responsive element (CRE) palindromic octanucleotide, TGACCTCA. The best characterized members of this gene family include CREB-1, CREB-2, ATF-1,ATF- 2,ATF-3and ATF-4. these transcription factors share highly-related COOH terminal leucine zipper demerization and basic DNA bindings but are highly divergent in their amino terminal domains. Although each of the ATF/CREB proteins bind CREs in their homodimeric form, in cerain instances they also bind as heterodimers, both within the ATF/CREB family and with members of the AP-1 transcription factor family. It has recentlybeen shown that protein kinase A- mediated CREB phosphorylation results in its binding to a 265kDa nuclear protein designated CBP (CREB-binding protein), which may reprecent a CREB co-activator.
Source Note: Polyclonal p-CREB-1 antibody was produced from sera of mice immunized with synthetic protein containing residues surrounding ser133. Reconstitution: A quick spin of the vial followed by reconstitution in 0.01M PBS pH 7.4 or other diluents. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CREB1 are readily searchable from our website. Different antibodies against the same target such as CREB1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.