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anti-CREB1 antibody product blog

Posted on 2015-06-11 22:36:31 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; CREB1 / CREB; anti-CREB1 antibody; CREB1;
The CREB1 creb1 (Catalog #MBS241375) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal (IgG) to Human CREB1 / CREB reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s CREB1 / CREB can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB).
IHC-P (10 ug/ml), WB (1:500 - 1:1000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti.. Researchers should empirically determine the suitability of the CREB1 creb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The CREB1 creb1 product has the following accession number(s) (GI #4758054) (NCBI Accession #NP_004370.1) (Uniprot Accession #P16220). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rabbit Polyclonal (IgG) to Human CREB1 / CREB with the following immunoassay(s):
Immunohistochemistry (IHC) (Anti-CREB1 / CREB antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Immunohistochemistry (IHC) CREB1.

Western Blot (WB) (Western blot of extracts from 293, HT29 and HeLa cells using CREB(Ab-133) antibody.)
Western Blot (WB) CREB1.

CREB (cAMP response element binding protein1), a DNA binding protein, is a member of the leucine zipper family of transcription factors. CREB interaction with DNA requires phosphorylation at Ser 119 by activated cAMP protein kinase A. Once phosphorylated CREB binds to an 8 base enhancer know as the cAMP response element (CRE) containing a conserved core sequence TGACG. CREB also interacts with CBP (CREB binding protein) and RNA polymerase II when phosphorylated on Ser 133.

Target Species: Human
Immunogen Description: Synthetic peptide from human CREB1 / CREB. Immunogen Type: Synthetic peptide
Immunogen: CREB1 / CREB antibody was raised against synthetic peptide from human CREB1 / CREB.
Antigen Modification: Ser133. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CREB1 are readily searchable from our website. Different antibodies against the same target such as CREB1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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