anti-COL1A1 antibody product blog
Tags: Antibody; Collagen Type I; COL1A1; Polyclonal Antibody; anti-COL1A1 antibody;
The COL1A1 col1a1 (Catalog #MBS534202) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Collagen Type I antibody (biotin) reacts with Reacts with Human, Bovine, Rat, and Mouse. Less than 0.1% cross-reactivity with Type II, III, IV, V, or VI Collagens. and may cross-react with other species as described in the data sheet. MyBioSource\'s Collagen Type I can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB).IP: 1:100
IHC: 1:50-1:200
ELISA: 1:3,000-1:6,000
WB: 1:3,000-1:6,000. Researchers should empirically determine the suitability of the COL1A1 col1a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The COL1A1 col1a1 product has the following accession number(s) (GI #119615037) (NCBI Accession #EAW94631.1) (Uniprot Accession #P02452). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Collagen Type I antibody (biotin) with the following immunoassay(s):
Western Blot (WB) (Western Blot of Collagen I antibody. Lane 1: Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced). Lane 2: PPARg-transduced cell lysates. Load: 100 ug per lane. Protein staining shown below each blot depicts equal protein loading. anti-Collagen I antibody at 0.22 ug/10 ml for overnight at 4 deg C. horseradish peroxidase-conjugated rabbit secondary antibody at 1 ug/10 ml for overnight at 4 deg C. Block: TBS with 5% Non-fat milk. Size: 138.9 kDa for Collagen I.)
Immunohistochemistry (IHC) (Tissue: Human Skin at pH9. Fixation: formalin fixed paraffin embedded. Collagen Type I antibody at 10 ug/mL for 1 h at RT. Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Collagen Type I is secreted in the extracellular matrix. Staining: Collagen Type I as precipitated brown signal (A) with hematoxylin purple nuclear counterstain. With corresponding negative conrol (B).)
Flow Cytometry (FC/FACS) (FACS analysis of mouse lung cells. Collagen type I antibody (biotin) detected with PE-conjugated CD45 and PE-conjugated collagen type I secondary antibody.)
Collagen is a group of naturally occurring proteins found, in nature, exclusively in animals, especially in the flesh and connective tissues of mammals. It is the main component of connective tissue, and is the most abundant protein in mammals, making up about 25% to 35% of the whole-body protein content. Type-I collagen is the most abundant collagen of the human body. It is present in scar tissue, the end product when tissue heals by repair. It is found in tendons, the endomysium of myofibrils and the organic part of bone.