anti-Cofilin 1 (N-terminus) antibody product blog
Tags: Antibody; Polyclonal Antibody; Cofilin 1 (N-terminus); anti-Cofilin 1 (N-terminus) antibody;
The Cofilin 1 (N-terminus) n/a (Catalog #MBS472015) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Cofilin 1 (N-terminus) reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s Cofilin 1 (N-terminus) can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot.ELISA: 1:2000
Immunocytochemistry: 1:50
Western Blot: 1:1000. Researchers should empirically determine the suitability of the Cofilin 1 (N-terminus) n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Cofilin 1 (N-terminus) with the following immunoassay(s):
Testing Data
Testing Data 2
Members of the ADF/cofilin (AC) family are actin-severing proteins that regulate actin remodeling during cellular events such as cell migration, cytokinesis, phagocytosis, endocytosis, axon development, and immune cell activation. In mammals, there are three members of the AC family, muscle-specific cofilin (cofilin 2), non-muscle cofilin (cofilin 1), and ADF. In humans, cofilin 1 and ADF have 72% identity, with the major amino acid differences found in the C-terminal region. Regulation of cofilin activity can occur through serine phosphorylation. Activation of cofilin kinases, LIMK1 or LIMK2, leads to phosphorylation of cofilin at serine 3. This phosphorylation disrupts cofilin binding to actin in vitro and in vivo. Multiple phosphatases, PP1, PP2A, PP2B, slingshot, and chronophin can dephosphorylate Ser-3 and activate actin binding. Thus, Ser-3 phosphorylation is a major site for the regulation of cofilin activity.
Immunogen: A synthetic peptide (coupled to KLH) corresponding to amino acid residues at the N-terminus of human Cofilin 1. This sequence has 100% homology with similar regions of rat and mouse Cofilin 1, and has 3 amino acid differences from human Cofilin 2. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Cofilin 1 (N-terminus) are readily searchable from our website. Different antibodies against the same target such as Cofilin 1 (N-terminus) may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.