anti-CNKSR1 antibody product blog
Tags: Antibody; AIF; Polyclonal Antibody; anti-CNKSR1 antibody; CNKSR1;
The CNKSR1 cnksr1 (Catalog #MBS150089) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AIF Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s AIF can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC).AIF antibody can be used for detection of AIF by Western blot at 0.5 - 2 mug/mL. A 67 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 5 mug/mL. Researchers should empirically determine the suitability of the CNKSR1 cnksr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CNKSR1 cnksr1 product has the following accession number(s) (GI #50400606) (NCBI Accession #Q969H4.1) (Uniprot Accession #O95381). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the AIF Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of AIF in K562 with AIF antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.)
Immunocytochemistry (IHC) (Immunocytochemistry of AIF in K562 cells with AIF antibody at 5 μg/mL.)
AIF Antibody: Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF). Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection. AIF is highly conserved between human and mouse and widely expressed.
Conjugate: Unconjugated
Immunogen: AIF antibody was raised against a 14 amino acid peptide near the carboxy terminus of human AIF.
Buffer: AIF Antibody is supplied in PBS containing 0.02% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CNKSR1 are readily searchable from our website. Different antibodies against the same target such as CNKSR1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.