anti-CLU antibody product blog
Tags: Antibody; Clusterin; CLU; Monoclonal Antibody; anti-Clu antibody;
The CLU clu (Catalog #MBS200054) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Clusterin antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Clusterin can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC).Recommended dilution range for Western blot is 1:500 ~ 1:2,000 and immunohistochemistry analysis is 1:100~200.
Recommended starting dilution for Western blot is 1:1,000 and Immunohistochemistry is 1:100. Researchers should empirically determine the suitability of the CLU clu for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CLU clu product has the following accession number(s) (GI #355594753) (NCBI Accession #NP_001822.3) (Uniprot Accession #P10909). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Clusterin antibody with the following immunoassay(s):
Western Blot (WB) (Cell lysates of U87MG (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Clusterin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Immunohistochemistry (IHC) (Paraffin embedded sections of human renal cell carcinoma tissue were incubated with anti-human Clusterin (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB))
Immunofluorescence (IF) (ICC/IF analysis of Clusterin in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Clusterin antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Clusterin has been implicated in numerous processes including active cell death, immune regulation, cell adhesion and morphological transformation. Clusterin (previousiy named SP-40) is a glycoprotein, 80kDa comprising two distinct 40kDa polypeptide chains. It shows marked similarity, both physicochemically and in immunohistological distribution, to the single chain, 80kDa, Sprotein, an additional component of the SC5b-9 complex. Clusterin is a secreted glycoprotein of which the exact function remains a matter for speculation.
Antigen Species: Human
Immunogen: Recombinant human Clusterin (1-333aa) purified from E. coli. Clone Note: Anti-human Clusterin mAb, clone 1A11, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Clusterin protein. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CLU are readily searchable from our website. Different antibodies against the same target such as CLU may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.