anti-CLEC5A antibody product blog
Tags: Antibody; Monoclonal Antibody; CLEC5A; anti-CLEC5A antibody;
The CLEC5A clec5a (Catalog #MBS609584) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CLEC5A (C-type Lectin Domain Family 5 Member A, C-type Lectin Superfamily Member 5, Myeloid DAP12-associating Lectin 1, MDL-1, CLECSF5, MDL1) (APC) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CLEC5A can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS).Suitable for use in Flow Cytometry.
Dilution: Flow Cytometry: 10ul labels 1-2.5x10e5 cells. Validated using whole blood monocytes.
Flow Cytometry Protocol: 1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction.
2. After blocking, add 10ul of C5839-75 to 1-2.5x10e5 cells and incubate for 30 minutes at RT.
3. Remove unbound antibody by washing the cells twice in Flow Cytometry Staining Buffer (F4568-10). Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer.
4. Resuspend the cells in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with APC-labeled mouse IgG2B antibody. Researchers should empirically determine the suitability of the CLEC5A clec5a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CLEC5A clec5a product has the following accession number(s) (GI #10281669) (NCBI Accession #NP_037384.1) (Uniprot Accession #Q9NY25). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the CLEC5A (C-type Lectin Domain Family 5 Member A, C-type Lectin Superfamily Member 5, Myeloid DAP12-associating Lectin 1, MDL-1, CLECSF5, MDL1) (APC) with the following immunoassay(s):
Flow Cytometry (FC/FACS) (Detection of MDL‑1/CLEC5A in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with (A) S1013-58B and (B) MBS609584 (filled histogram) or isotype control antibody (open histogram).)
CLEC5A is a type II membrane protein belonging to the C-type lectin superfamily. CLEC5A is expressed on monocytes/macrophages, dendritic cells, and NK cells and contributes to cellular activation through its association with DAP12.
Immunogen: Recombinant corresponding to the extracellular domain of human CLEC5A. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CLEC5A are readily searchable from our website. Different antibodies against the same target such as CLEC5A may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.