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anti-CLDN1 antibody product blog

Posted on 2015-01-21 05:07:37 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; CLDN1; anti-CLDN1 antibody; Claudin-1;
The CLDN1 cldn1 (Catalog #MBS301154) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Anti-Human Claudin 1 Polyclonal Antibody reacts with Human, Mouse
Predicted Reactivity: Bovine, Dog, Pig, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Claudin-1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Western Blot (WB).
Immunohistochemistry Procedure
Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:200. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 10 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system.
Immunohistochemistry Dilution: 1:200
IHC Positive Control: Skin, Breast Carcinoma, Ovarian Carcinoma

Western Blot
Recommended Starting Protocol: Dilute the antibody 1:25. Incubate for 1 hour at room temperature. The dilution is an estimate; actual results may differ because of variability in methods and protocols. Optimal dilution and procedure should be determined by the end user.
Western Blot Positive Control: HeLa Cell Lysate. Researchers should empirically determine the suitability of the CLDN1 cldn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The CLDN1 cldn1 product has the following accession number(s) (GI #10863887) (NCBI Accession #NP_066924.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rabbit Anti-Human Claudin 1 Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of HeLa cell lysate with claudin 1 antibody)
Western Blot (WB) CLDN1.

Immunohistochemistry (IHC) (Human skin stained with anti-claudin 1 antibody)
Immunohistochemistry (IHC) CLDN1.

Claudin 1 is a member of claudin family and a transmembrane protein associated with tight junctions. Tight junctions are specialized regions of cell to cell contact; made up of network of strands to act as a molecular gasket for preventing the leakage of ions, water etc. between cells. They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different claudin composition. Claudin-1 protein expression is up-regulated in various tumors such as carcinomas of the prostate, breast, and colon.

Immunogen: Synthetic peptide derived from C-terminus of human claudin-1 peptide.
Control Tissue: Breast Carcinoma, Ovarian Carcinoma, Skin
Cellular Localization: Cell Junction, Cytoplasm, Membrane
Entrez Gene Code: 9076
Pathway: Cell Junctions.
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