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anti-CHUK antibody product blog

Posted on 2013-12-16 19:33:25 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; anti-CHUK antibody; CHUK; IkB alpha;
The CHUK chuk (Catalog #MBS625007) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IkB alpha, phosphorylated (Ser32/Ser36) (NF-kappa-B Inhibitor alpha, I-kappa-B-alpha, IkB-alpha, IkappaBalpha, Major Histocompatibility Complex Enhancer-binding Protein MAD3, NFKBIA, IKBA, MAD3, NFKBI) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s IkB alpha can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC).
Suitable for use in Immunohistochemistry.
Dilution: Immunohistochemistry:1:50-1:100. Researchers should empirically determine the suitability of the CHUK chuk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The CHUK chuk product has the following accession number(s) (GI #62241001) (NCBI Accession #NP_001269.3) (Uniprot Accession #O15111). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Three major forms of IKB like molecules have been identified and each is characterised by multiple copies of ankyrin repeats. IKB alpha and IKB beta appear to be the major regulatory forms of IKB in most cells. These proteins interact with p65 or cRel containing forms of NFkB and block nuclear import by masking the nuclear localisation sequences of NFkB. The activation of NFkB involves the inducible phosphorylation and subsequent degradation of IKB. Immunoblotting easily detects the hyperphosphorylated forms of IKB alpha, but not phosphorylated IKB beta. Interestingly, IKB alpha and IKB beta mediate different NFkB responses. IkB alpha appears to control more transient activation of NFkB in response to an inducer, while IKB beta controls a persistent response. Bcl3 interacts with p50 and p52 containing forms of NFkB, but rather than being an inhibitor it appears to function to stimulate transcription. The degradation of IKB is confirmed by immunoblotting.

CHUK chuk

Immunogen: Synthetic phosphopeptide corresponding to human IkB alpha around the phosphorylation site of serine 32/36 (H-D-SP-G-L-D-SP -M-K). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CHUK are readily searchable from our website. Different antibodies against the same target such as CHUK may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Blood, Bone, Brain, Embryonic Tissue, Heart, Kidney, Liver, Lung, Muscle, Skin tissues are correlated with this protein.
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