anti-Chlamydia sp., Multi-epitope Cocktail antibody product blog
Tags: Antibody; Monoclonal Antibody; Chlamydia sp., Multi-epitope Cocktail; anti-Chlamydia sp., Multi-epitope Cocktail antibody;
The Chlamydia sp., Multi-epitope Cocktail n/a (Catalog #MBS600422) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s Chlamydia sp., Multi-epitope Cocktail can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF).Immunohistochemistry: Detects Chlamydia species in specimens after fixation with methanol/acetone (1:1). Also suitable for paraformaldehyde-fixed tissue sections and cell culture.
Optimal dilutions to be determined by the researcher.
Other applications not tested. Researchers should empirically determine the suitability of the Chlamydia sp., Multi-epitope Cocktail n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
The multi-epitope cocktail detects the elementary bodies (Ebs), reticulate bodies (RBs), intermediate forms, chlamydial inclusions and specific cell-associated antigen(s) directly in samples. The conjugate is used for direct immunofluorescence staining combining three specific monoclonal antibodies (mab) conjugated to fluorescein isothiocyanate (FITC). One mab is specific for the genus-specific epitope located on the Chlamydia LPS and identifies all the 15 known serovars of C. trachomatis as well as C. psittaci and C. pneumoniae by displaying bright fluorescence of intracellular inclusions and pin-point shaped extracellular organisms as well as free cell-associated chlamydial antigen(s). A second mab reacts with an epitope on the Chlamydia outer membrane complex protein (60 kD) and shows preferential fluorescence around the periphery of individual RBs of all serotypes of C. trachomatis and of C. psittaci strains; this antibody also stains the EBs of C-complex serovars as pin-point shaped structures. The third mab identifies a species-specific epitope on the major outer membrane pro- tein (40 kD) and shows brilliant fluorescence with EBs, RBs and cytoplasmic inclusions of B-complex serovars of C. trachomatis, whereas C-complex serovars show medium to weak fluorescence. The combination of these 3 specific mabs provides uniform and intense staining of all the stages in the developmental cycle of the known C. trachomatis serovars (A�C, D�K, L1�L3) and C. psittaci and C. pneumoniae strains. A positive cross-reactivity was also reported for Chlamydophila pecorum.
Immunogen: Chlamydia antigen. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Chlamydia sp., Multi-epitope Cocktail are readily searchable from our website. Different antibodies against the same target such as Chlamydia sp., Multi-epitope Cocktail may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.