anti-CFLAR antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-CFLAR antibody; CFLAR; FLIP;
The CFLAR cflar (Catalog #MBS150432) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FLIP Antibody reacts with Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s FLIP can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC).FLIP antibody can be used for the detection of FLIP by Western blot at 0.5 mug/mL. 55 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 5 mug/mL. Researchers should empirically determine the suitability of the CFLAR cflar for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CFLAR cflar product has the following accession number(s) (GI #2253679) (NCBI Accession #AAC51622) (Uniprot Accession #O15519). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the FLIP Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of FLIP in K562 cell lysate with FLIP antibody at (A) 1 and (B) 2 μg/ml.)
Immunocytochemistry (IHC) (Immunocytochemistry of FLIP in 3T3 cells with FLIP antibody at 5 μg/mL.)
FLIP Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) containing adapter molecules and members of the ICE/CED-3 protease family.Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP respectively. The human FLIP was also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH, CLARP and MRIT. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by all known death receptors CD95, DR3, TRAIL-R and TNFR1.
Conjugate: Unconjugated
Immunogen: FLIP antibody was raised against a 17 amino acid peptide near the carboxy terminus of mouse FLIP.
Buffer: FLIP Antibody is supplied in PBS containing 0.02% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CFLAR are readily searchable from our website. Different antibodies against the same target such as CFLAR may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.