anti-CFLAR antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-CFLAR antibody; CFLAR; FLIP;
The CFLAR cflar (Catalog #MBS150357) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FLIP Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s FLIP can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF).FLIP can be used for detection of FLIP by Western blot at 1:1000 dilution. 35 kDa and 25 kDa bands can be detected. Antibody can also be used for immunocytochemistry starting at 2 ug/mL. For immunofluorescence start at 10 ug/mL.
Optimal dilutions for each application to be determined by the researcher. Researchers should empirically determine the suitability of the CFLAR cflar for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CFLAR cflar product has the following accession number(s) (GI #2253679) (NCBI Accession #AAC51622) (Uniprot Accession #O15519). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the FLIP Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of FLIP in total cell lysates from HeLa (H), Jurkat (J), THP-1 (T), A431 (A), K562 (K) and NIH3T3 (N) cells with FLIP antibody at 1:1000 dilution.)
Immunocytochemistry (IHC) (Immunocytochemistry staining of HeLa cells using FLIP antibody at 2 μg/mL.)
Immunofluorescence (IF) (Immunofluorescence of FLIP in HeLa cells with FLIP antibody at 10 μg/mL.)
Immunofluorescence (IF) (Immunofluorescence of FLIP in Jurkat cells with FLIP antibody at 20 μg/mL.
Red: FLIP Antibody (2055)
Blue: DAPI staining)
Immunocytochemistry (ICC) (Immunocytochemistry of FLIP in Jurkat cells with FLIP antibody at 2 μg/mL.)
FLIP Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)-containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and c-FLIPs, respectively. The human FLIPs were also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH, CLARP and usurpin. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by all known death receptors CD95, DR3, TRAIL-R and TNFR1. Four splice variants of c-FLIPs have been identified and termed FLIPalpha, beta, gamma, and delta, respectively.
Conjugate: Unconjugated
Immunogen: FLIP antibody was raised against a 19 amino acid peptide near the carboxy terminus of human FLIP.
The immunogen is located within amino acids 180-230 of FLIP
Buffer: FLIP Antibody is supplied in PBS containing 0.02% sodium azide.