anti-CF150 antibody product blog
Tags: Antibody; Polyclonal Antibody; CF150; anti-CF150 antibody;
The CF150 n/a (Catalog #MBS6001732) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CF150, ID (MB21D1, C6orf150, Protein MB21D1, Mab-21 domain-containing protein 1) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CF150 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS).Suitable for use in Western Blot, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the CF150 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CF150 n/a product has the following accession number(s) (GI #515465858) (NCBI Accession #EPL14397.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the CF150, ID (MB21D1, C6orf150, Protein MB21D1, Mab-21 domain-containing protein 1) with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of MDA-MB231 cell line lysates (35ug/lane) using MBS6001732.)
Western Blot (WB) (Western Blot analysis of lysate from THP-1 cell line (20ug) using MBS6001732 (1:1000). IgG (HRP) goat anti-rabbit at 1:10,000 was used as the secondary antibody.)
Western Blot (WB) (Western Blot analysis of lysates from MCF-7, HT-29, THP-1 cell line, human spleen, human cerebellum tissue lysate (from left to right, 20ug/lane), using MBS6001732 (1:1000/lane). IgG (HRP) goat anti-rabbit at 1:10,000 was used as the secondary antibody.)
Testing Data (Overlay histogram showing U-2OS cells stained with MBS6001732 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% BSA to block non-specific protein-protein interactions followed by MBS6001732 (1:25) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1:400 for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10e6 cells) used under the same conditions. Acquisition of >10,000 events was performed.)
The exact function of C6orf150 remains unknown.
Immunogen: CF150 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 273-303 amino acids from the Central region of human CF150. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CF150 are readily searchable from our website. Different antibodies against the same target such as CF150 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.