anti-CDKN2A antibody product blog
Tags: Antibody; Polyclonal Antibody; CDKN2A; anti-CDKN2A antibody;
The CDKN2A cdkn2a (Catalog #MBS150506) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CDKN2A Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s CDKN2A can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).CDKN2A antibody can be used for detection of CDKN2A by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the CDKN2A cdkn2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CDKN2A cdkn2a product has the following accession number(s) (GI #4502749) (NCBI Accession #NP_000068) (Uniprot Accession #P42771). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the CDKN2A Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of CDKN2A in mouse colon tissue lysate with CDKN2A antibody at (A) 1 and (B) 2 μg/mL.)
Immunohistochemistry (IHC) (Immunohistochemistry of CDKN2A in rat colon tissue with CDKN2A antibody at 5 μg/mL.)
Immunofluorescence (IF) (Immunofluorescence of CDKN2A in Human Colon tissue with CDKN2A antibody at 20 μg/mL.)
Immunohistochemistry (IHC) (Immunohistochemistry of CDKN2A in human colon tissue with CDKN2A antibody at 10 μg/mL.)
Immunofluorescence (IF) (Immunofluorescence of CDKN2A in rat colon tissue with CDKN2A antibody at 20 μg/ml.
Red: CDKN2A Antibody (4211)
Blue: DAPI staining)
CDKN2A Antibody: The CDKN2A locus gives rise to 2 distinct transcripts from different promoters. The transcripts have been designated p16(INK4A) and p14(ARF). This chromosomal region undergoes a number of inversions, translocations, heterozygous deletions, and homozygous deletions in a variety of malignant cell lines including those from glioma, non-small cell lung cancer, leukemia, and melanoma. Deletion of the region containing CDKN2A is found in more than half of all melanoma cell lines. Conversely, transfection of CDKN2A suppressed the growth of two independent mesothelioma cell lines, suggesting that inactivation of the CDKN2 gene is an essential step in the etiology of malignant mesotheliomas. CDKN2A induces a G1 cell cycle arrest by inhibiting the phosphorylation of the Rb protein by the cyclin-dependent kinases CDK4 and CDK6. CDKN2A is expressed as at least three distinct isoforms.
Conjugate: Unconjugated
Immunogen: CDKN2A antibody was raised against an 18 amino acid synthetic peptide from near the amino terminus of human CDKN2A.
Buffer: CDKN2A Antibody is supplied in PBS containing 0.02% sodium azide.