anti-CDC23 antibody product blog
Tags: Antibody; Polyclonal Antibody; APC8; anti-CDC23 antibody; CDC23;
The CDC23 cdc23 (Catalog #MBS150979) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The APC8 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s APC8 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC).APC8 antibody can be used for detection of APC8 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunocytochemistry starting at 5 mug/mL. Researchers should empirically determine the suitability of the CDC23 cdc23 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CDC23 cdc23 product has the following accession number(s) (GI #254763423) (NCBI Accession #Q9UJX2.3) (Uniprot Accession #Q9UJX2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the APC8 Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of APC8 in K562 cell lysate with APC8 antibody at (A) 1 and (B) 2 μg/mL.)
Immunocytochemistry (IHC) (Immunocytochemistry of APC8 in K562 cells with APC8 antibody at 5 μg/mL.)
APC8 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC8 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. In Drosophila, silencing of APC8 results in developmental delay and pupal lethality with elevated levels of apoptosis, high mitotic index, and delayed or blocked mitosis.
Conjugate: Unconjugated
Immunogen: APC8 antibody was raised against a 17 amino acid synthetic peptide near the center of human APC8.
Buffer: APC8 Antibody is supplied in PBS containing 0.02% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CDC23 are readily searchable from our website. Different antibodies against the same target such as CDC23 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.