anti-CDC2 (pY15) (CDK1) antibody product blog
Tags: Antibody; CDC2 (pY15) (CDK1); anti-CDC2 (pY15) (CDK1) antibody;
The CDC2 (pY15) (CDK1) n/a (Catalog #MBS462185) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti CDC2 (Paired Y15), Non-phospho reacts with Human, Chicken, Bovine and may cross-react with other species as described in the data sheet. MyBioSource\'s CDC2 (pY15) (CDK1) can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot, Immunohistochemistry.Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 1:200. Researchers should empirically determine the suitability of the CDC2 (pY15) (CDK1) n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CDC2 (pY15) (CDK1) n/a product has the following accession number(s) (GI #17136606) (NCBI Accession #NP_476797.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rabbit anti CDC2 (Paired Y15), Non-phospho with the following immunoassay(s):
Testing Data
Cyclins and cyclin-dependent kinases (CDKs) control cell cycle progression by phosphorlating regulatory proteins. These cyclin-related proteins appear to affect cell structure and function independent of the cell cycle. Cyclins and CDKs have a role in the development and maintenance of cell- and tissue-restricted properties of differentiated cells. CDC2 (Cell Division Cycle 2), also known as CDK1 (Cyclin Dependent kinase 1), is a member of the CDK family of serine/threonin kinases. It is a highly conserved serine protein kinase that plays a key role in regulation of the cell cycle. The phosphorylation of CDC2 at Y15 and T14 during the G2 phase of the cell cycle inhibits CDC2 activity, while the dephosphorylation of Y15 and T14 by CDC25 phosphatase during the late G2 restores its activity.
Antigen Preparation: Cell division control protein 2 (CDC2); Cell division protein kinase 2 (CDK2), CDK1, CDK3, MGC11195, CDC28AA synthetic peptide surrounding to the epitope -GTYGV- without a phosphorylation site at Tyr15 of human CDC2 protein. This sequence is identical among human, mouse, rat, bovine and chicken species. Positive Control: A431 cell lysate
Cellular Location: Nuclear. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CDC2 (pY15) (CDK1) are readily searchable from our website. Different antibodies against the same target such as CDC2 (pY15) (CDK1) may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.