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anti-CD8 antibody product blog

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Posted on 2014-04-19 00:12:39 by mybiosource_staff
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Tags: Antibody; CD8; Monoclonal Antibody; anti-CD8 antibody;
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The CD8 cd8a (Catalog #MBS684124) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti - CD8 reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CD8 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) - Formalin/Paraffin. Researchers should empirically determine the suitability of the CD8 cd8a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The CD8 cd8a product has the following accession number(s) (GI #116035) (NCBI Accession #P01732.1) (Uniprot Accession #P01732). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti - CD8 with the following immunoassay(s):
Immunohistochemistry (IHC) (CD8 expression in T-lymphocytes of the palatine tonsil. Formalin fixed, paraffin embedded human tissue (4 ?msection) stained with anti -CD8 (MBS684124) monospecific clonal antibody)
Immunohistochemistry (IHC) CD8.

Immunogen: Peptide derived from C-terminal region of human CD8
Storage Buffer: 20 mM Tris-HCI, pH 8.0
Stabilizer: 20 mg/ml BSA
Preservative: 0.05% NaN3
Immunogen: Peptide derived from C-terminal region of human CD8. Antibody recognizes the epitope between Pro218 - Tyr234.
Positive control: human tonsil tissue
Cellular localization: membrane. IHC-P PROTOCOL–INSTRUCTION MANUAL: 1.Deparaffinize the section in 3 changes of xylene, 5 minutes each.
2.Wash the section in 96%, 80% and 70% ethyl alcohol for 10 minutes each.
3.Rinse in distilled water.
4.Block the endogenous peroxidase by incubating the tissue in 3% hydrogen peroxide (H2O2) for 10 minutes.
5.Wash in distilled water.
6.For antigen retrieval: immerse the slide in Tris-EDTA buffer, pH 9.0,0.05% Tween-20*, and incubatein water bath at 96-98ºC for 20-25 minutes.
7.Transfer the slide to room temperature and let it cool down for 15 minutes in the buffer used in the step #6
8.Rinse in distilled water.
9.Wash in 0.05 M Tris-HCl, pH 7.6 buffer supplemented with 0.2% of Tween-20 (buffer A) for 5 minutes.
10.READY TO USE (RTU): Incubate the section with primary antibody (ready to use)for 1 hour in a closed wet chamber.
11.Wash twice 5 minutes with buffer A.
12.Apply the secondary antibody (the protocol depends on the supplier), and proceed to standard mmunohistochemistry protocol.
13.Wash twice 5 minutes with buffer A.
14.Apply the chromogen (DAB), 1 -3 minutes.
15.Wash in water –10 minutes.
16.Stain in hematoxylin for 5 minutes.
17.Wash in water –10 minutes.8.Dehydrate the section in 2 changes of 96% ethyl alcohol for 5 minutes each.
19.Wash the section in 2 changes of xylene for 2 minutes each.
20.Mount the slide for observation.

PRECAUTIONS: 1.We strongly recommend to use DB Primary Antibody Diluent eventually alternative diluent (containing protease free BSA at the concentrations ? 1mg/ml) for dilution of concentrated antibodies, otherwise the warranty might be oided.
2.Centrifuge the vial before use.3.Intended for professional In Vitro Diagnostic use in laboratories.
4.Do not use after expiration date stamped on vial label.
5.Avoid contamination of the reagent.
6.Any discrepancies in the recommended procedures stated in the working protocol may affect the final results.
7.The reagent contains sodium azide (NaN3) which is highly toxic in higher concentrations. The concentration in the reagent (0.05%) is not considered as hazardous.
8.Disposal of waste material must be conducted in accordance with local regulations.
9.Wear appropriate Personal Protective Equipment to avoid contact with eyes and skin.
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