anti-CD8 ALPHA antibody product blog
Tags: Antibody; Monoclonal Antibody; CD8 ALPHA; anti-CD8 ALPHA antibody;
The CD8 ALPHA cd8a (Catalog #MBS211034) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD8 ALPHA can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS).Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: 1/5; Maximum Dilution: 1/10. Researchers should empirically determine the suitability of the CD8 ALPHA cd8a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CD8 ALPHA cd8a product has the following accession number(s) (GI #13928726) (NCBI Accession #NP_113726.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MOUSE ANTI RAT CD8 ALPHA:FITC with the following immunoassay(s):
Testing Data (Published customer image: Qualitative and quantitative flow cytometric analysis of lymphocyte populations in draining lymph nodes. A: Representative FACS plot of CD4+ CD8+ staining used to count T-helper cells, cytotoxic T-cells and CD4+ CD8+ double positive T-lymphocytes. Events acquired: 2x105. B: FACS plot example for B-cell detection. C: Representative FACS plot for NK cell assessment. NK-T cell were confirmed by CD3 expression (not shown). Bar diagrams: Cumulative results for the quantification of major and minor lymphocyte populations in draining LN of cornea transplanted animals. An asterisk (*) indicates statistical significance at p=0.05 determined by Mann -Whitney U-Test. Allo-Tx-d7 - animals allo-grafted and analyzed at day 07 post op, n=6; allo-Tx-rej - animals displaying allo rejection of grafted corneas analyzed after the onset of rejection, n=5; syn-Tx-d7 - syngeneically grafted animals analyzed at day 7 post-op, n=3; syn-Tx-LT - syn-grafted long-term survivors analyzed at the end of the observation period at day 42; n=3.Maenz M, Morcos M, Ritter T. A comprehensive flow-cytometric analysis of graft infiltrating lymphocytes, draining lymph nodes and serum during the rejection phase in a fully allogeneic rat cornea transplant model. Mol Vis. 2011 Feb 8;17:420-9.)
Testing Data (Staining of rat peripheral blood cells with Mouse anti Rat CD8 Alpha Chain:Alexa Fluor 488 )
Testing Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD25 antibody, red in An and Mouse anti Rat CD8, green in B. C is the merged image with nuclei counterstained blue using DAPI. High power)
Testing Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD25 antibody, red in An and Mouse anti Rat CD8, green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power)
Testing Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD25 antibody, red in An and Mouse anti Rat CD8, green in B. C is the merged image with nuclei counterstained blue using DAPI. Low powe)
Testing Data (Published customer image: CD4 and CD8 T cell infiltration. Photos show SN sections of an animal of the cell death group immunostained with antibody against CD4 (An and C) and CD8 (B and D). The small panels show insets in A (C) and in B (D) at higher magnification. Scale: 50 um, applies to A -B, 10 um applies to C -D. (E) Graph shows average (dash) and individual numbers of CD4+ cells found in one SN section per animal of each group plotted per time. Two-way ANOVA [F (8,42) = 4.1, p = 0.001 effect of group and time interaction] followed by Tukey HSD post-hoc analysis. ## or � p)
Testing Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD25 antibody, red in An and Mouse anti Rat CD8, green in B. C is the merged image with nuclei counterstained blue using DAPI. High power)
Testing Data (Immunofluoescence staining of rat lymph node cryosection with Mouse anti Rat CD11b antibody, red in An and Mouse anti Rat CD8, green in B. C is the merged image with nuclei counter-stained in blue using DAPI. High power)
Testing Data (Published customer image:Analysis of graft-infiltrating T cells. Activated CD25+ T cells and CD4+ and CD8+ T cell subsets were stained at the time points of corneal allograft rejection and calculated within the graft. A, C, and E show representative histological staining for CD25, CD4, and CD8 in grafts of treated and control animals, respectively. CD25+ (B) and CD4+ (D) cells infiltrated to a statistically significantly stronger extent in 3.2.3-treated animals when compared to control treated animals (*p)
Testing Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD163 antibody, red an An and Mouse anti Rat CD8 MCA48), green in B. C is the merged image with nuclei counter-stained blue using DAPI. High power)