|The CD66e ceacam5 (Catalog #MBS215174) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD66e can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS).
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10. Researchers should empirically determine the suitability of the CD66e ceacam5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CD66e ceacam5 product has the following accession number(s) (GI #612407802) (NCBI Accession #NP_001278413.1) (Uniprot Accession #P06731). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MOUSE ANTI HUMAN CD66e:FITC with the following immunoassay(s):
Testing Data (Published customer image: Immunostaining and cell surface expression of epithelial/pancreatic markers and overexpressed proteins in PaCa 5061 cells. 2A. The formaldehyde fixed and agar embedded cells were immunostained for the presence of pancreatic cancer markers CA 19-9 and CEA respectively, as well as for cytokeratin as epithelial cell marker proteins (upper panel). Several on RNA-level overexpressed genes (Microarray) were chosen. To confirm protein overexpression of selected amplified genes in PaCa 5061 cells immunostaining was performed for Mucin4, EGFR and CEACAM 6 (lower panel). 2B. FACS profiles of PaCa 5061 cells. Cell surface expression of CD44, EpCAM, EGFR, CEACAM1, CEACAM5 and CEACAM6 were obtained with specific antibodies as in materials and methods. Each histogram shows cell surface expression of the corresponding marker (filled curves) and the irrelevant, isotype-matched antibody (open curves). From: Kalinina T, GngÂr C, Thieltges S, MÂller-Krull M, Penas EM, Wicklein D, Streichert T, Schumacher U, Kalinin V, Simon R, Otto B, Dierlamm J, Schwarzenbach H, Effenberger KE, Bockhorn M, Izbicki JR, Yekebas EF. Establishment and characterization of a new human pancreatic adenocarcinoma cell line with high metastatic potential to the lung. BMC Cancer. 2010 Jun 16;10:295.)
Mouse anti Human CD66e antibody, clone C365D3 (NCRC23) recognizes human Carcinoembryonic antigen-related cell adhesion molecule 5, also known as CD66e, carcinoembryonic antigen, Meconium antigen 100, CEA or CEACAM5. CD66e is a 702 amino acid ~77 kDa GPI anchored membrane protein containing 7 Ig-like domains. Mouse anti Human CD66e antibody, clone C365D3 does not cross-react with normal cross-reacting antigen (CD66c), or with biliary g lycoprotein (CD66a) as indicated by binding assays (Price 1988, note: in this study Mouse anti Human CD66e antibody, clone C365D3 is designated as clone 6 (from author)).