anti-CD62E/CD62P antibody product blog
Tags: Antibody; Monoclonal Antibody; CD62E/CD62P; anti-CD62E/CD62P antibody;
The CD62E/CD62P sele (Catalog #MBS216652) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD62E/CD62P can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen*, ELISA (EIA), Flow cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB)*.Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Immunohistology - Frozen: Minimum Dilution: 1ug/ml; Maximum Dilution: 10ug/ml; Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. MyBioSource recommends the use of acetone fixation for frozen sections.
ELISA: Maximum Dilution: 10ug/ml
Flow Cytometry: Maximum Dilution: 10ug/ml
Western Blotting: Application Note: Clone 1.2B6 recognizes human CD62E/CD62P only under non-reducing conditions. Researchers should empirically determine the suitability of the CD62E/CD62P sele for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CD62E/CD62P sele product has the following accession number(s) (GI #187960042) (NCBI Accession #NP_000441.2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MOUSE ANTI HUMAN CD62E/CD62P with the following immunoassay(s):
Testing Data (Staining of thrombin activated human peripheral blood platelets with Mouse anti Human CD62E/CD62P: FITC (MBS211116))
Testing Data (Staining of thrombin activated human peripheral blood platelets with Mouse anti Human CD62E/CD62P (MBS213185))
Mouse anti Human CD62E/CD62P antibody, clone 1.2B6 recognizes the human CD62E and CD62P cell surface antigens. Although previously thought to recognize only human CD62E, recent data (Goda et al. 2003) shows that Mouse anti Human CD62E/CD62P antibody, clone 1.2B6 also recognizes human CD62P, binding to a common epitope shared by these members of the selectin family.
Perservative Stabilisers: 0.09% Sodium Azide
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant. Immunogen: Human E-Selectin (ELAM-1)
Histology Positive Control Tissue: Human Tonsil
Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
Buffer Solution: Phosphate buffered saline
Target Species: Human. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD62E/CD62P are readily searchable from our website. Different antibodies against the same target such as CD62E/CD62P may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.