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anti-CD61 antibody product blog

Posted on 2013-10-16 01:21:12 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; CD61; anti-CD61 antibody;
The CD61 itgb3 (Catalog #MBS210510) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD61 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS).
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
Flow Cytometry: Maximum Dilution: Neat. Researchers should empirically determine the suitability of the CD61 itgb3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The CD61 itgb3 product has the following accession number(s) (GI #47078292) (NCBI Accession #NP_000203.2) (Uniprot Accession #P05106). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MOUSE ANTI HUMAN CD61:Biotin with the following immunoassay(s):
Testing Data (Staining of human peripheral blood platelets with Mouse anti Human CD61:RPE (MBS213869))
Testing Data CD61.

Testing Data (Staining of human peripheral blood platelets with Mouse anti Human CD61:FITC (MBS211080))
Testing Data CD61.

Testing Data (Staining of human peripheral blood platelets with Mouse anti Human CD61:Alexa Fluor 647 (MBS213131A647))
Testing Data CD61.

Mouse anti Human CD61 antibody, clone PM6/13 recognizes a 105kD glycoprotein, which is the integrin beta 3 chain. This molecule associates with either the alpha IIb integrin (CD41) or the alpha V integrin (CD51) at the cell surface. CD61 is expressed on platelets and megakaryocytes in association with CD41, and on endothelial cells, monocytes, platelets and osteoclasts in association with CD51. CD61 is a receptor for fibrinogen, fibronectin, vWF, vitronectin and thrombospondin. Mouse anti Human CD61 antibody, clone PM6/13 has been reported to inhibit binding of fibronectin to activated human platelets. In functional studies it can be used to inhibit agonist induced platelet aggregation.

Perservative Stabilisers: 0.09% Sodium Azide
1% Bovine Serum Albumin
Preparation: Purified IgG prepared by ion exchange chromatography. Immunogen: Platelet plasma membrane
Fusion Partners: Spleen cells from immunised mice were fused with cells of the mouse SP2/0 myeloma cell line.
Buffer Solution: Phosphate buffered saline
Target Species: Human. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD61 are readily searchable from our website. Different antibodies against the same target such as CD61 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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