|The CD49d itga4 (Catalog #MBS221512) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD49d can be used in a range of immunoassay formats including, but not limited to, Immunohistology Paraffin*.
Immunohistology - Paraffin: Minimum Dilution: 15; Maximum Dilution: 25ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Researchers should empirically determine the suitability of the CD49d itga4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CD49d itga4 product has the following accession number(s) (GI #67191027) (NCBI Accession #NP_000876.3) (Uniprot Accession #P13612). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the RABBIT ANTI HUMAN CD49d (C-TERMINAL) with the following immunoassay(s):
Testing Data #1 (Staining of formalin fixed, paraffin embedded human thymic medulla with Rabbit anti Human CD49d (MBS221512))
Rabbit anti Human CD49d polyclonal antibody detects an epitope within the C-terminal region of human CD49d, also known as integrin alpha-4 or VLA-4 subunit alpha. CD49d is a ~150kDa single pass type 1 transmembrane glycoprotein with seven FG-GAP repeats, characteristic of alpha integrins, in its extracellular domain. CD49d can be proteolytically cleaved to yield framents of 80 and 70kDa (Hemler et al. 1987). CD49d associates with either CD29 to form VLA-4 or with Integrin beta-7 to form The Peyer patches-specific homing receptor LPAM-1, involved in the lymphocyte migration and homing to gut-associated lymphoid tissue (Sackstein 2006) through its interaction with MadCam-1, preferentially expressed on Peyer\'s patch high endothelial venules and postcapillary venules in lamina propria (Briskin et al. 1997). VLA-4 is expressed on lymphocytes, monocytes, mast cells, macrophages, basophils and eosinophils, and has key roles in inflammation, cellular adhesion and cellular activation. LPAM-1 is expressed mainly on lymphocytes, and plays a key role in lymphocyte recruitment to sites of inflammation.
Perservative Stabilisers: 0.1% Sodium Azide (NaN3). Histology Positive Control Tissue: Thymus, Medulla.
Buffer Solution: Phosphate buffered saline
Antiserum Preparation: Antisera to human CD49d were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography.
Target Species: Human. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD49d are readily searchable from our website. Different antibodies against the same target such as CD49d may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.