anti-CD48 antibody product blog
Tags: Antibody; Monoclonal Antibody; CD48; anti-CD48 antibody;
The CD48 cd48 (Catalog #MBS520170) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Rat CD48 Biotin (Clone OX-45) (mouse IgG1) reacts with Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s CD48 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC).FLOW CYTOMETRY ANALYSIS:
Method:
1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte�-Rat cell separation medium.
2. Wash 2 times.
3. Resuspend the cells to a concentration of 2x107 cells/ml in media A. Add 50 ul of this suspension to each tube (each tube will then contain 1x106 cells, representing 1 test).
4. To each tube, add 0.2 ug* of MBS520170.
5. Vortex the tubes to ensure thorough mixing of antibody and cells.
6. Incubate the tubes for 30 minutes at 4�C.
7. Wash 2 times at 4�C.
8. Add 100 ul of secondary antibody (Streptavidin-FITC) at a 1:500 dilution.
9. Incubate tubes at 4�C for 30 - 60 minutes (It is recommended that tubes are protected from light since most fluorochromes are light sensitive).
10. Wash 2 times at 4�C.
11. Resuspend the cell pellet in 50 ul ice cold media B.
12. Transfer to suitable tubes for flow cytometric analysis containing 15 ul of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA.
Media:
A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 ul of 2M sodium azide in 100 mls).
B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 ul of 2M sodium azide in 100 mls). Researchers should empirically determine the suitability of the CD48 cd48 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CD48 cd48 product has the following accession number(s) (GI #21361571) (NCBI Accession #NP_001769.2) (Uniprot Accession #P09326). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-Rat CD48 Biotin (Clone OX-45) (mouse IgG1) with the following immunoassay(s):
Testing Data
This anti-rat CD45 (Blast-1) monoclonal antibody recognizes a rat cell surface glycoprotein of 45 kDa that is present on a wide variety of hematopoietic cells and on endothelial cells. The antigen is identical to the mouse BCM1 antigen. This antibody inhibits allogeneic mixed lymphocyte reactions using lymph node cells as responders and spleen cells as stimulators. CD48 has recently been identified as a ligand of the NK cell inhibitory receptor CD244. This antibody is suitable for flow cytometry.
Immunogen: Rat T cell blasts (stimulated purified T helper cells with allogeneic irradiated rat spleen cells)
Donor: BALB/c spleen
Fusion Partner: Myeloma cell line NSO/1.