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anti-CD45 antibody product blog

Posted on 2015-07-16 16:08:53 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; CD45; anti-CD45 antibody;
The CD45 ptprc (Catalog #MBS216363) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD45 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS).
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10. Researchers should empirically determine the suitability of the CD45 ptprc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The CD45 ptprc product has the following accession number(s) (GI #158138490) (NCBI Accession #NP_001103357.1) (Uniprot Accession #P04157). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MOUSE ANTI RAT CD45:FITC with the following immunoassay(s):
Testing Data (Staining of rat spleen cells with Mouse anti Rat CD45:FITC)
Testing Data CD45.

Testing Data (Staining of rat peripheral blood lymphocytes with Mouse anti Rat CD45 (MBS213009))
Testing Data CD45.

Testing Data (Staining of rat spleen cells with Mouse anti Rat CD45: RPE (MBS213827))
Testing Data CD45.

Testing Data (Staining of rat spleen cells with Mouse anti Rat CD45:Alexa Fluor 488 )
Testing Data CD45.

Mouse anti Rat CD45 antibody, clone OX-1 recognizes CD45, also known as the leucocyte common antigen (LCA). The leucocyte common antigen consists of a family of heavily glycosylated membrane glycoproteins of molecular weight 180 - 240kDa. Antibodies recognising a common epitope on all of these isoforms are termed CD45, whilst those recognising only individual isoforms are termed CD45RA, CD45RO etc. OX-1 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes. CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction. This product is routinely tested in flow cytometry on rat splenocytes.

Perservative Stabilisers: 0.09% Sodium Azide
1% Bovine Serum Albumin. Immunogen: Rat thymocyte membrane glycoproteins.
Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
Buffer Solution: Phosphate buffered saline
Target Species: Rat. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD45 are readily searchable from our website. Different antibodies against the same target such as CD45 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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