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anti-CD40 antibody product blog

Posted on 2014-11-22 13:17:43 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; CD40; anti-CD40 antibody;
The CD40 cd40 (Catalog #MBS520120) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-Mouse CD40, Biotin (Clone 3/23) (rat IgG2a) reacts with Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s CD40 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the CD40 cd40 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The CD40 cd40 product has the following accession number(s) (GI #23312371) (NCBI Accession #NP_690593.1) (Uniprot Accession #P25942). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-Mouse CD40, Biotin (Clone 3/23) (rat IgG2a) with the following immunoassay(s):
Testing Data
Testing Data CD40.

anti-mouse monoclonal antibody reacts with CD40 which is expressed on B-lymphocytes and on a subset of both CD4+ and CD8+ T cells in the adult (but not neonatal) spleen1. The level of CD40 on B cells is enhanced following activation buy LPS1. The interactions of CD40 with its ligand CD40L are responsible for generating humoral responses, germinal center formation, and generating B cell memory2. Stimulating cells with immobilized 3/23 mAb increases expression of CD86 (B7.2) on B cells1,3. Though the 3/23 mAb itself is a weak mitogen, it produces a synergistic proliferative B cell response with IL-4 or with mitogenic antibodies against surface Ig 1,3. Costimulation with 3/23 mAb and IL-4 rescues B cells from apoptosis induced by hypercross-linking of sIgM or sIgD3. This antibody is suitable for use in flow cytometry. Other applications of this antibody include IHC of acetonefixed frozen sections2.

Presentation: 100 ug or 300 ug Biotin conjugated Ig buffered in PBS, 0.1% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD40 are readily searchable from our website. Different antibodies against the same target such as CD40 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene.
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