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anti-CD34 antibody product blog

Posted on 2014-03-06 21:07:09 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; CD34; anti-CD34 antibody;
The CD34 cd34 (Catalog #MBS211207) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD34 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS), Immunoprecipitation (IP), Immunohistology Paraffin.
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Immunohistology - Paraffin: Minimum Dilution: 1/20; Maximum Dilution: 1/200. Researchers should empirically determine the suitability of the CD34 cd34 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The CD34 cd34 product has the following accession number(s) (GI #19526792) (NCBI Accession #NP_598415.1) (Uniprot Accession #Q64314). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the RAT ANTI MOUSE CD34:Low Endotoxin with the following immunoassay(s):
Testing Data (Staining of mouse bone marrow with Rat anti Mouse CD34: Alexa Fluor 488 (MBS212332A488))
Testing Data CD34.

Testing Data (Staining of mouse bone marrow cells with Rat anti Mouse CD34:FITC (MBS210779))
Testing Data CD34.

Testing Data (Staining of WEHI cells with Rat anti Mouse CD34 (MBS212333))
Testing Data CD34.

Testing Data (Staining of WEHI cells with Rat anti Mouse CD34: RPE (MBS213675))
Testing Data CD34.

Testing Data (Staining of mouse bone marrow with Rat anti Mouse CD34: Alexa Fluor 647 (MBS212332A647))
Testing Data CD34.

Rat anti Mouse CD34 antibody, clone MEC14.7 recognizes the murine CD34 cell surface antigen, which is expressed by endothelial cells and by haematopoietic stem cells. This antibody recognizes a neuraminidase sensitive epitope. As in the human system, CD34 antibodies in the mouse demonstrate slightly different staining patterns depending on their fine specificity. Rat anti Mouse CD34 antibody, clone MEC14.7 appears to recognize a subset of the stem cell population recognized by clone RAM34, and it is thought that this is due to differences in the epitope recognized by the two antibodies.

Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. Immunogen: T-end.1, a pMT transformed endothelial cell line
Buffer Solution: Phosphate buffered saline
Target Species: Mouse. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD34 are readily searchable from our website. Different antibodies against the same target such as CD34 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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