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anti-CD31 antibody product blog

Posted on 2014-10-19 11:41:32 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; CD31; anti-CD31 antibody;
The CD31 pecam1 (Catalog #MBS210660) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD31 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS).
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100. Researchers should empirically determine the suitability of the CD31 pecam1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The CD31 pecam1 product has the following accession number(s) (GI #75832033) (NCBI Accession #NP_113779.1) (Uniprot Accession #Q3SWT0). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MOUSE ANTI RAT CD31:FITC with the following immunoassay(s):
Testing Data (Immunohistochemical staining of rat spleen cryosection with Mouse anti Rat CD31 (MBS212104))
Testing Data CD31.

Testing Data (Immunohistochemical staining of rat brain cryosection with Mouse anti Rat CD31 (MBS212105))
Testing Data CD31.

Mouse anti Rat CD31 antibody, clone TLD-3A12 recognizes rat PECAM-1 (CD31), a 661 amino acid type 1 transmembrane protein expressed primarily on endothelial cells, platelets and leucocytes. Clone TLD-3A12 has been shown to partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen (Stevenson, K.S. et al.2009). This clone has been reported as suitable for use in IHC on formalin-fixed paraffin-embedded sections pre-treated with 0.2M boric acid, pH7.0. (Wilson et al. 2007). Mouse anti Rat CD31, clone TLD-3A12 has been shown to be cross-reactive with endothelial cells derived from rhesus macaque (Maclean et al. 2001).

Perservative Stabilisers: 0.09% Sodium Azide
1% Bovine Serum Albumin
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. Buffer Solution: Phosphate buffered saline
Target Species: Rat. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD31 are readily searchable from our website. Different antibodies against the same target such as CD31 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Breast Neoplasms, Cardiovascular Diseases, Heart Diseases, Inflammation, Liver Diseases, Liver Neoplasms, Lung Diseases, Lung Neoplasms, Necrosis, Neoplasms, Experimental are some of the diseases may be linked to MOUSE ANTI RAT CD31:FITC.
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