Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Blog  rightarrow anti-CD3 antibody  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • anti-TNK2 antibody blog
 • RANK recombinant protein blog
 • anti-SOCS7 antibody blog
 • anti-SDHB antibody blog
 • anti-FGF23 antibody blog
 • SNX5 peptide blog
 • SNW1 elisa kit blog
 • TEX38 sirna blog
 • ANGA elisa kit blog
 • Tissue, Genomic DNA, Nude Mouse Adult Normal, Kidney, BioGenomics genomic dna blog
 • anti-Amyloid, beta (1-43) C-terminal antibody blog
 • anti-NKRF antibody blog
 • Procalcitonin recombinant protein blog
 • anti-albumin antibody, AAA elisa kit blog
 • DECR1 blocking peptide blog
 • E2 elisa kit blog
 • Hemagglutinin-Influenza A Virus H7N7 Netherlands 219/03 recombinant protein blog
 • TMEM8C sirna blog
 • anti-LYPLA1 antibody blog
 • CHFR peptide blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • 4 hydroxybenzoate polyprenyltransferase, mitochondrial (COQ2... ELISA Kit
 • ABRI - ITM2B Antibody
 • Heat Shock Protein 70 (HSP70) ELISA Kit
 • MDM1 cDNA Clone
 • Protein Wnt-7b (WNT7B) ELISA Kit
 • G0-G1switch 2 (G0S2) ELISA Kit
 • TNFAIP3 Interacting Protein 1 (TNIP1) Recombinant Protein
 • Carnitine palmitoyltransferase I (CPT 1) ELISA Kit
 • v-ets erythroblastosis virus E26 oncogene homolog 2 (ETS2) Antibody
 • Paxillin (PXN) Antibody
 • Polyethylene Glycol Antibody
 • Granylocyte colony stimulating factor (GCSF) Antibody
 • UBE2Z Antibody
 • Glucan endo-1,3-alpha-glucosidase agn1 (agn1) Recombinant Protein
 downarrow more ...
separator

anti-CD3 antibody product blog

separator
Posted on 2014-07-08 01:46:02 by mybiosource_staff
Share it on ...     
separator
separator
Tags: Antibody; CD3; Monoclonal Antibody; anti-CD3 antibody;
separator
The CD3 cd3e (Catalog #MBS219803) is an Antibody produced from Hamster and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD3 can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Flow cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB).
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR.
Flow Cytometry: Minimum Dilution: 1/25; Maximum Dilution: 1/200. Researchers should empirically determine the suitability of the CD3 cd3e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The CD3 cd3e product has the following accession number(s) (GI #6680886) (NCBI Accession #NP_031674.1) (Uniprot Accession #P22646). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the HAMSTER ANTI MOUSE CD3 with the following immunoassay(s):
Testing Data (Mouse splenocytes stained with Hamster anti Mouse CD3:AlexaFluor488 (MBS214442A488))
Testing Data CD3.

Testing Data (Mouse splenocytes stained with Hamster anti Mouse CD3 (MBS214442))
Testing Data CD3.

Hamster anti Mouse CD3 antibody, clone 145-2C11 detects CD3 epsilon (CD3e), a 20kDa transmembrane protein also known as CD3 or T3. CD3e is a member of the CD3 complex which consists of four subunits, gamma, delta, epsilon and zeta, and these are associated to the T cell receptor (TCR). TCR plays a critical role in T cell development and function, and is responsible for ligand recognition. It interacts non-covalently with the CD3 dimers delta/epsilon, gamma/epsilon and zeta/zeta which transduce signals from the TCR into the cell. CD3e is primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. Hamster anti Mouse CD3 antibody, clone 145-2C11 is useful for in vitro blocking and activation assays, as well as apoptosis induction and in vitro T cell depletions.

Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. Immunogen: H-2Kb - specific mouse cytotoxic T lymphocyte clone BM10-37.
Buffer Solution: Phosphate buffered saline
Target Species: Mouse. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD3 are readily searchable from our website. Different antibodies against the same target such as CD3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions