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anti-CD289 antibody product blog

Posted on 2015-01-11 13:36:21 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; CD289; anti-CD289 antibody;
The CD289 tlr9 (Catalog #MBS224156) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The GOAT ANTI MOUSE CD289 (N-TERMINAL) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CD289 can be used in a range of immunoassay formats including, but not limited to, Immunohistology Paraffin, Western Blot (WB).
Immunohistology - Paraffin: Minimum Dilution: 1:250;
Western Blotting: Minimum Dilution: 1:1000. Researchers should empirically determine the suitability of the CD289 tlr9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The CD289 tlr9 product has the following accession number(s) (GI #8394456) (NCBI Accession #NP_059138.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the GOAT ANTI MOUSE CD289 (N-TERMINAL) with the following immunoassay(s):
Testing Data #1 (Staining of paraffin embedded mouse thymus with Goat anti mouse CD289 (MBS224156))
Testing Data #1 CD289.

Testing Data #2 (Staining of paraffin embedded human spleen with Goat anti mouse CD289 (MBS224156))
Testing Data #2 CD289.

Goat anti Mouse CD289 antibody recognises murine Toll-like receptor 9, also known as CD289 or TLR9. CD289 is an 1032 amino acid ~120 kDa endosomal single pass type I transmembrane glycoprotein with a number of leucine rich repeats and a single TIR domain (UniProt: Q9NR96). Goat anti Mouse CD289 antibody recognizes an epitope within the N-terminal (NT) region of CD289, a member of the toll-like receptor family, which triggers MyD88 and TRAF6, resulting in NF-kappaB activation, cytokine secretion and the inflammatory response. CD289 acts as a receptor for unmethylated cytidine-phosphate-guanosine dinucleotides in bacterial DNA (CpG-DNA), playing a primary role in pathogen recognition and activation of innate immunity andhas been shown to play a role in defence against systemic mouse cytomegalovirus infection. Goat anti Mouse CD289 antibody is reported as suitable for use in immunocytochemistry on acetone fixed cells.

Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
0.1% Bovine Serum Albumin. Histology Positive Control Tissue: Spleen, thymus or tonsil
Buffer Solution: Phosphate buffered saline
Antiserum Preparation: Antiserum to mouse CD289 (NT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Target Species: Mouse. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD289 are readily searchable from our website. Different antibodies against the same target such as CD289 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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