anti-CD284 antibody product blog
Tags: Antibody; Monoclonal Antibody; CD284; anti-CD284 antibody;
The CD284 tlr4 (Catalog #MBS212470) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI HUMAN CD284 reacts with Dog, Guinea Pig, Pig, Rhesus Monkey and may cross-react with other species as described in the data sheet. MyBioSource\'s CD284 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB).Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/25. Researchers should empirically determine the suitability of the CD284 tlr4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CD284 tlr4 product has the following accession number(s) (GI #4507533) (NCBI Accession #NP_003257.1) (Uniprot Accession #O00206). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MOUSE ANTI HUMAN CD284 with the following immunoassay(s):
Testing Data (Staining of human peripheral blood monocytes with Mouse anti Human CD284: Alexa Fluor 488 (MBS212470A488))
Testing Data (Staining of human peripheral blood monocytes with Mouse anti Human CD284: Alexa Fluor 647 (MBS212470A647))
Testing Data (Staining of human peripheral blood monocytes with Mouse anti Human CD284: FITC (MBS210833))
Testing Data (Staining of human peripheral blood monocytes with Mouse anti Human CD284)
Testing Data (Staining of U937 cells with Mouse anti Human CD284:RPE (MBS213711))
Testing Data (Staining of human peripheral blood monocytes with Mouse anti Human CD284: Low Endotoxin (MBS211211))
Mouse anti Human CD284 antibody, clone HTA125 recognizes the human Toll like receptor 4 (TLR4) cell surface antigen. TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule. TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS. This antibody has been demonstrated to block activation of monocytes with LPS. MyBioSource recommend the use of MBS211211 for this purpose.
Perservative Stabilisers: 0.09% Sodium Azide
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. Immunogen: Ba/F3 cell line expressing TLR4 (CD284)
Fusion Partners: Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Buffer Solution: Phosphate buffered saline
Target Species: Human. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD284 are readily searchable from our website. Different antibodies against the same target such as CD284 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.