anti-CD276 antibody product blog
Tags: Antibody; Monoclonal Antibody; CD276; anti-CD276 antibody;
The CD276 cd276 (Catalog #MBS850476) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD276 Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CD276 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA).WB: 1:500-1:2000
IF: 1:200-1:1000
FC: 1:200-1:400
ELISA: 1:10000. Researchers should empirically determine the suitability of the CD276 cd276 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CD276 cd276 product has the following accession number(s) (GI #67188443) (NCBI Accession #NP_001019907.1) (Uniprot Accession #Q5ZPR3). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the CD276 Antibody with the following immunoassay(s):
Western Blot (WB) (Figure 1: Western blot analysis using CD276 mAb against HEK293 (1) and CD276-hIgGFc transfected HEK293 (2) cell lysate.)
Immunohistochemistry (IHC) (Figure 2: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues (left) and ovarian cancer tissues (right) using CD276 mouse mAb with DAB staining.)
Flow Cytometry (FC/FACS) (Figure 3: Flow cytometric analysis of PC-3 cells using CD276 mouse mAb (green) and negative control (purple).)
Costimulatory B7 molecules (e.g., B7-1, or CD80; MIM 112203) signal through CD28 (MIM 186760) family molecules such as CD28, CTLA4 (MIM 123890), and ICOS (MIM 604558). May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.
Immunogen: Purified recombinant fragment of human CD276 expressed in E. Coli. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD276 are readily searchable from our website. Different antibodies against the same target such as CD276 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.