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anti-CD252 antibody product blog

Posted on 2013-10-02 18:41:35 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; CD252; anti-CD252 antibody;
The CD252 tnfsf4 (Catalog #MBS215318) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD252 can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Flow cytometry (FC/FACS).
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Researchers should empirically determine the suitability of the CD252 tnfsf4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The CD252 tnfsf4 product has the following accession number(s) (GI #6678473) (NCBI Accession #NP_033478.1) (Uniprot Accession #P43488). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the RAT ANTI MOUSE CD252 with the following immunoassay(s):
Testing Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD252 antibody, clone OX-89 (MBS212367) followed by Goat anti Rat IgG antibody (MBS235195). High power)
Testing Data CD252.

Testing Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD252 antibody, clone OX-89 (MBS212367) followed by Goat anti Rat IgG antibody (MBS235195). Low power)
Testing Data CD252.

Rat anti Mouse CD252 antibody, clone OX-89 recognizes mouse OX40 (CD134) ligand, which is otherwise known as CD252. CD252 expression has been shown to be restricted to activated dendritic cells, B cells and vascular endothelium. Rat anti Mouse CD252 antibody, clone OX-89 has been reported to inhibit OX40/OX40L interaction (Malstrom et al. 2001).

Perservative Stabilisers: 0.09% Sodium Azide
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. Immunogen: Rat CD4 and mouse CD134 ligand Chimaeric protein
Fusion Partners: Spleen cells from immunised PVG rats were fused with cells of the NS1 myeloma cell line.
Buffer Solution: Phosphate buffered saline
Target Species: Mouse. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD252 are readily searchable from our website. Different antibodies against the same target such as CD252 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Cardiovascular Diseases, Heart Diseases, Inflammation, Kidney Diseases, Lung Diseases, Lupus Erythematosus, Systemic, Myocardial Infarction, Myocardial Ischemia, Necrosis, Neoplasms, Experimental are some of the diseases may be linked to RAT ANTI MOUSE CD252.
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