anti-CD25 antibody product blog
Tags: Antibody; Monoclonal Antibody; CD25; anti-CD25 antibody;
The CD25 pmp22 (Catalog #MBS370153) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD25 reacts with Human, Mouse and may cross-react with other species as described in the data sheet.The CD25 pmp22 product has the following accession number(s) (GI #55904) (Uniprot Accession #P25094). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the CD25 with the following immunoassay(s):
Dilution Information
Immunohistochemistry (IHC) (Inset: IHC of CD25 on a FFPE Lung Tissue)
Recommended IHC Protocol
CD25 is the alpha chain of the IL-2 receptor. It is a Type I transmembrane protein present on activated T-cells, activated B-cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. It is expressed in most B-cell neoplasms, some Acute Non-lymphocytic Leukemias, and Neuroblastomas. Expression of CD25 is a reliable tool for distinguishing neoplastic mast-cell aggregates from reactive proliferations, and has, therefore, recently become a minor criterion for the identification of Systemic Mastocytosis (SM). Anti-CD25 antibodies have also been useful in identifying mast cells in skin biopsies in the setting of Urticaria Pigmentosa, which is predictive of Systemic Mastocytosis. Quantitation of regulatory T-cells (Treg) in the setting of hepatocellular carcinoma has been used as an independent predictive factor for tumor recurrence after hepatic resection for HCC. Also, the percentage of tumor-infiltrating CD25+FOXP3+ regulatory T-cells among tumor cells, inside tumor parenchyma and at its periphery are significantly higher in recurrent Cutaneous Melanoma than in Non-recurrent Melanoma.
Control: Mastocytosis, Tonsil, Small Bowel
Localization: Cytoplasmic, Membranous
Immunogen: Recombinant protein corresponding to the external domain of the Interleukin-2 Receptor molecule.
Precautions: 1. This product contains sodium azide (NaN3), a toxic chemical which may react with plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent sodium azide build-up.
2. Ensure proper handling procedures are used with reagent. Always wear proper laboratory equipment such as laboratory coat and gloves when handling reagents.
3. Unused solution should be disposed of according to local and federal regulations.
4. Do not ingest reagent. If reagent ingested, contact a poison control centerimmediately.
5. For complete recommendations for handling biological specimens please refer to the CDC document, �Guidelines for Safe Work Practices in Human and Animal Medical Laboratories� (5).
Specimen Preparation: Paraffin sections:
The antibody can be used on formalin-fixed paraffin-embedded (FFPE) tissue sections. Ensure tissue undergoes appropriate fixation to ensure best results. Pre-treatment of tissues with heat-induced epitope retrieval (HIER) is recommended using ImmunoDNA Retriever with Citrate, ImmunoDNA Retriever with EDTA or ImmunoDNA Digestor Tissue should remain hydrated via use of Immuno/DNA Washer solutions.
Frozen sections and cell preparations:
The antibody can be used for labeling acetone-fixed frozen sections and acetone-fixed cell preparations.